RFLP for the human prothrombin (F2) gene

A TaqI RFLP for the human arylsulfatase A gene.

A frequent Hindlll RFLP of the human fibrorrertin gene ( FNl )

SOURCE/DESCRIPTION: The probe used is a Hindlll/BamHI fragment of 1.4kb from the fibronectin recombinant, pFH6 (Kornblihtt, A.R. et al.). POLYMORPHISM: Hindlll (A/AGCTT) (New England, Bio-Labs, FRG) detects invariant bands at 4.75 and 1.7kb in addition to a simple two-allele polymorphism defined by the presence of a band of 7.1kb (F2) alternatively with two bands of 3.7 and 3.4kb (Fl) (Figure 1...

MspI RFLP in the human fumarylacetoacetate hydrolase (FAH) gene.

Radioimmunoassay for human prothrombin.

I describe a radioimmunoassay for human prothrombin, with use of a double-antibody technique. Antiserum raised in rabbits was absorbed with Al(OH)3 and heated to 56 degrees C for 30 min. 125I-labeled prothrombin retaining more than 90% of its biological activity was prepared by the iodine monochloride method. The mean concentration of prothrombin in plasma of 12 normal individuals was 100 +/- 2...

Human prothrombin activation.

Human prothrombin has been purified from American Red Cross Factor IX concentrates. Studies of the activation of the human prothrombin with the use of sodium dodecyl sulfate electrophoretic analysis of activation products indicated that human prothrombin activation is similar to bovine prothrombin activation. Molecular weight analysis of human prothrombin and intermediated by sodium dodecyl sul...